Infrared spectroscopy with visible light

Novel surface vibrational spectroscopy: infrared-infrared-visible sum-frequency generation.

A novel type of surface vibrational sum-frequency generation spectroscopy is presented that enables a highly specific measurement of the coupling of molecules on surfaces. With this doubly vibrationally resonant technique, two-dimensional vibrational spectroscopy of molecules on surfaces becomes possible. The technique is demonstrated for the C-O stretch vibration of CO on a ruthenium (001) sur...

Fusion of Infrared and Visible-Light Images

Visible light optical coherence correlation spectroscopy.

Optical coherence correlation spectroscopy (OCCS) allows studying kinetic processes at the single particle level using the backscattered light of nanoparticles. We extend the possibilities of this technique by increasing its signal-to-noise ratio by a factor of more than 25 and by generalizing the method to solutions containing multiple nanoparticle species. We applied these improvements by mea...

Analytical Imaging of Cultural Heritage by Synchrotron Radiation and Visible Light - near Infrared Spectroscopy

Imaging is an important tool for analyzing cultural heritage. Due to its delicate nature, the analysis presents numerous technical challenges, probably the most important of which is its requirement for non-destructive and non-invasive investigation. In this study, two techniques used in the analysis of cultural heritage are presented. The first one, synchrotron radiation x-ray fluorescence, is...

Monitoring light-induced structural changes of Channelrhodopsin-2 by UV-visible and Fourier transform infrared spectroscopy.

Channelrhodopsin-2 (ChR2) is a microbial type rhodopsin and a light-gated cation channel that controls phototaxis in Chlamydomonas. We expressed ChR2 in COS-cells, purified it, and subsequently investigated this unusual photoreceptor by flash photolysis and UV-visible and Fourier transform infrared difference spectroscopy. Several transient photoproducts of the wild type ChR2 were identified, a...